Liverpool midfielder Adam Lallana has been ruled out of England duty with a groin strain.
Lallana will miss the upcoming international fixtures against Spain and Switzerland.
The 30-year-old began Tuesday’s training session at St George’s Park with the rest of the 22-man squad. Shortly into the session, however, he pulled up with a potential injury. Lallana left the session, and underwent further tests.
England have since confirmed the Liverpool midfielder will return to his club for treatment. He will take no further part in the first UEFA Nations League matches.
Injury issues cost Lallana a place in the squad that reached the World Cup semi-finals during the summer.
Southgate’s squad is already down to 22 men due to Raheem Sterling withdrawing on Monday evening with a back problem.
The remaining players trained together for the first time since the World Cup on Tuesday morning. Fellow Liverpool player Dominic Solanke has also received a call up to the squad, in order to bolster numbers.
Those involved in Premier League action on Sunday left the session early, including rejuvenated Manchester United left-back Luke Shaw.